Aliases:	LGMN; Legumain; LGMN1; PRSC1; Protease, Cysteine, 1 (Legumain) ; Asparaginyl Endopeptidase; Protease, Cysteine 1; EC 3.4.22.34; Cysteine Protease 1; AEP
Background:	UniProt Entry: Q99538;NCBI Gene Entry: 5641
Application Information	Molecular Weight: Predicted, 49 kDa, observed, 36,57 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2095 Spncies Reactivity: Human, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM)
Immunogen	A synthesized peptide derived from human Legumain
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Legumain expression in HeLa cells using Legumain antibody 1:2,000. Green, isotype control; red, Legumain.

Western blotting analysis using anti-Legumain antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Legumain antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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