Aliases:	LIG4; DNA Ligase 4; Polydeoxyribonucleotide Synthase [ATP] 4; DNA Ligase IV; Ligase IV, DNA, ATP-Dependent; Polynucleotide Ligase; DNA Repair Enzyme; DNA Joinase; EC 6.5.1.1; Sealase; LIG4S
Background:	UniProt Entry: P49917;NCBI Gene Entry: 3981
Application Information	Molecular Weight: Predicted, 104 kDa, observed, 103 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2515 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human DNA Ligase IV
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of DNA ligase 4 expression in HeLa cells using DNA ligase 4 antibody 1:2,000. Green, isotype control; red, DNA ligase 4.

Immunocytochemical staining of Hela cells with DNA ligase 4 antibody 1:1,000. Nuclei were stained blue with DAPI; DNA ligase 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using anti-DNA ligase 4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-DNA ligase 4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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