Aliases:	LMAN1; Lectin, Mannose Binding 1; ERGIC53; ERGIC-53; MCFD1; FMFD1; F5F8D; MR60; Gp58; Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 53; ER-Golgi Intermediate Compartment 53 KDa Protein; Intracellular Mannose-Specific Lectin MR60; Protein ERGIC-53; Coagulation Factor V-Factor VIII Combined Deficiency; Lectin, Mannose-Binding, 1; Lectin Mannose-Binding 1; GP58
Background:	UniProt Entry: P49257;NCBI Gene Entry: 3998
Application Information	Molecular Weight: Predicted, 58 kDa, observed, 53 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2835 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human LMAN1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of LMAN1 expression in HepG2 cells using LMAN1 antibody 1:2,000. Green, isotype control; red, LMAN1.

Immunocytochemical staining of HepG2 cells with LMAN1 antibody 1:1,000. Nuclei were stained blue with DAPI; LMAN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-LMAN1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-LMAN1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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