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[KD-Validated] Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody
[KD-Validated] Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody
MTAP; Methylthioadenosine Phosphorylase; MSAP; S-Methyl-5'-Thioadenosine Phosphorylase; 5'-Methylthioadenosine Phosphorylase; MTA Phosphorylase; MTAPase; C86fus; Epididymis Secretory Sperm Binding Protein; Epididymis Luminal Protein 249; MeSAdo Ph
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  • NO.:KD-10617
    Gene name:MTAP
    React Species:Human; Mouse; Rat
    Applications:WB; FCM; IHC
    UniProt Entry:Q13126
    NCBI Gene Entry:4507
  • Goods click count:193
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Aliases: MTAP; Methylthioadenosine Phosphorylase; MSAP; S-Methyl-5'-Thioadenosine Phosphorylase; 5'-Methylthioadenosine Phosphorylase; MTA Phosphorylase; MTAPase; C86fus; Epididymis Secretory Sperm Binding Protein; Epididymis Luminal Protein 249; MeSAdo Phosphorylase; EC 2.4.2.28; HEL-249; DMSMFH; C86FUS; DMSFH; LGMBF; BDMF
Background: UniProt Entry: Q13126;NCBI Gene Entry: 4507
Application InformationMolecular Weight: Predicted, 31 kDa, observed, 29 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4560 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human MTAP
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols For general and specific antibody protocols please visit our website. Read all instructions before using this product.
Flow cytometric analysis of Methylthioadenosine phosphorylase expression in HT-1080 cells using Methylthioadenosine phosphorylase antibody 1:2,000. Green, isotype control; red, Methylthioadenosine phosphorylase.
Immunocytochemical staining of HT-1080 cells with Methylthioadenosine phosphorylase antibody 1:1,000. Nuclei were stained blue with DAPI; Methylthioadenosine phosphorylase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Methylthioadenosine phosphorylase antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.
Western blotting analysis using anti-Methylthioadenosine phosphorylase antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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