Aliases:	Mechanistic Target Of Rapamycin Kinase; RAFT1; RAPT1; Rapamycin And FKBP12 Target 1; Mammalian Target Of Rapamycin; FRAP1; FRAP2; FRAP; FK506-Binding Protein 12-Rapamycin Complex-Associated Protein 1; Mechanistic Target Of Rapamycin (Serine/Threonine Kinase); FK506 Binding Protein 12-Rapamycin Associated Protein 2; FKBP12-Rapamycin Complex-Associated Protein 1; Serine/Threonine-Protein Kinase MTOR; Rapamycin Associated Protein FRAP2; FKBP-Rapamycin Associated Protein; Mechanistic Target Of Rapamycin; Rapamycin Target Protein 1; FLJ44809; DJ576K7.1 (FK506 Binding Protein 12-Rapamycin Associated Protein 1); FK506 Binding Protein 12-Rapamycin Associated Protein 1; FKBP12-Rapamycin Complex-Associated Protein; Rapamycin Target Protein; EC 2.7.11.1; MTOR; SKS
Background:	UniProt Entry: P42345;NCBI Gene Entry: 2475
Application Information	Molecular Weight: Predicted, 289 kDa, observed, 255 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB200 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human mTOR
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of mTOR expression in HepG2 cells using mTOR antibody 1:2,000. Green, isotype control; red, mTOR.

Immunocytochemical staining of HepG2 cells with anti-mTOR antibody 1:1,000. Nuclei were stained blue with DAPI; mTOR was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-mTOR antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-mTOR antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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