Aliases:	NEK2; NIMA Related Kinase 2; NEK2A; NLK1; PPP1R111; RP67; NIMA (Never In Mitosis Gene A)-Related Kinase 2; Protein Phosphatase 1, Regulatory Subunit 111; Serine/Threonine-Protein Kinase Nek2; NimA-Related Protein Kinase; NimA-Like Protein Kinase 1; EC 2.7.11.1; Never In Mitosis A-Related Kinase; EC 2.7.11; HsPK 21; HSPK 21; HsPK21
Background:	UniProt Entry: P51955;NCBI Gene Entry: 4751
Application Information	Molecular Weight: Predicted, 52 kDa, observed, 48 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2085 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NEK2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of NEK2 expression in HepG2 cells using NEK2 antibody 1:2,000. Green, isotype control; red, NEK2.

Immunocytochemical staining of HepG2 cells with NEK2 antibody 1:1,000. Nuclei were stained blue with DAPI; NEK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-NEK2 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-NEK2 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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