Aliases:	NIMA Related Kinase 7; NIMA (Never In Mitosis Gene A)-Related Kinase 7; Serine/Threonine-Protein Kinase Nek7; Never In Mitosis A-Related Kinase 7; NimA-Related Protein Kinase 7; EC 2.7.11.34; EC 2.7.11.1
Background:	UniProt Entry: Q8TDX7;NCBI Gene Entry: 140609
Application Information	Molecular Weight: Predicted, 35 kDa, observed, 35 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2885 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NEK7
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of NEK7 expression in HepG2 cells using NEK7 antibody 1:2,000. Green, isotype control; red, NEK7.

Immunocytochemical staining of HepG2 cells with NEK7 antibody 1:1,000. Nuclei were stained blue with DAPI; NEK7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-NEK7 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-NEK7 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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