Aliases:	PRKCA; Protein Kinase C Alpha; PKCA; Protein Kinase C Alpha Type; EC 2.7.11.13; PKC-Alpha; PRKACA; PKC-A; Protein Kinase C, Alpha; Aging-Associated Gene 6; EC 2.7.11; PKCalpha; PKCI+/-; PKCα; AAG6
Background:	UniProt Entry: P17252;NCBI Gene Entry: 5578
Application Information	Molecular Weight: Predicted, 77 kDa, observed, 77 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4100 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-PKC alpha (T638)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Phospho-PKC alpha (T638) expression in HeLa cells using Phospho-PKC alpha (T638) antibody 1:2,000. Green, isotype control; red, Phospho-PKC alpha (T638).

Immunocytochemical staining of Hela cells with Phospho-PKC alpha (T638) antibody 1:1,000. Nuclei were stained blue with DAPI; Phospho-PKC alpha (T638) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Phospho-PKC alpha (T638) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Phospho-PKC alpha (T638) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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