Aliases:	PRKCA; Protein Kinase C Alpha; PKCA; Protein Kinase C Alpha Type; EC 2.7.11.13; PKC-Alpha; PRKACA; PKC-A; Protein Kinase C, Alpha; Aging-Associated Gene 6; EC 2.7.11; PKCalpha; PKCI+/-; PKCα; AAG6
Background:	UniProt Entry: P17252;NCBI Gene Entry: 5578
Application Information	Molecular Weight: Predicted, 77 kDa, observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4955 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM)
Immunogen	A synthesized peptide derived from human Phospho-PKC alpha (T497)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Phospho-PKC alpha (T497) expression in C2C12 cells using Phospho-PKC alpha (T497) antibody 1:2,000. Green, isotype control; red, Phospho-PKC alpha (T497).

Western blotting analysis using anti-Phospho-PKC alpha (T497) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Phospho-PKC alpha (T497) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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