Aliases:	Protein Kinase C; Alpha; EC 2.7.11.13;PKCA1; AAG6; PKC-A; Aging-Associated Gene 6; PKC-alpha; Protein Kinase C Alpha Type; PRKACA; EC 2.7.11
Background:	UniProt Entry: P17252;NCBI Gene Entry: 5578
Application Information	Molecular Weight: Predicted, 77 kDa, observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1690 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PKC alpha
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of PKC alpha expression in HepG2 cells using PKC alpha antibody 1:2,000. Green, isotype control; red, PKC alpha

Immunocytochemical staining of HT-1080 cells with PKC alpha antibody 1:1,000. Nuclei were stained blue with DAPI; PKC alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-PKC alpha antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-PKC alpha antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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