Aliases:	Ring Finger Protein 1; RNF1; RING-Type E3 Ubiquitin Transferase RING1; Really Interesting New Gene 1 Protein; E3 Ubiquitin-Protein Ligase RING1; Polycomb Complex Protein RING1; RING Finger Protein 1; EC 2.3.2.27; EC 6.3.2; RING1A
Background:	UniProt Entry: Q06587;NCBI Gene Entry: 6015
Application Information	Molecular Weight: Predicted, 42 kDa, observed, 50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB955 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human RING1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of RING1 expression in HepG2 cells using RING1 antibody 1:2,000. Green, isotype control; red, RING1.

Immunocytochemical staining of HepG2 cells with RING1 antibody 1:1,000. Nuclei were stained blue with DAPI; RING1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-RING1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

Western blotting analysis using anti-RING1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

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