Aliases:	Ras Homolog Family Member A; RHOH12; Rho12; ARH12; ARHA; Transforming Protein RhoA; Epididymis Secretory Sperm Binding Protein; Ras Homolog Gene Family, Member A; Aplysia Ras-Related Homolog 12; Small GTP Binding Protein RhoA; Rho CDNA Clone 12; Oncogene RHO H12; EC 3.6.5.2; EDFAOB; RHO12; H12
Background:	UniProt Entry: P61586;NCBI Gene Entry: 387
Application Information	Molecular Weight: Predicted, 22 kDa, observed, 22 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB865 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Rho A
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of RHOA expression in C2C12 cells using RHOA antibody 1:2,000. Green, isotype control; red, RHOA.

Immunocytochemical staining of C2C12 cells with anti-RHOA antibody 1:1,000. Nuclei were stained blue with DAPI; RHOA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-RHOA antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-RHOA antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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