Summary:

 

Purpose

 

Our Quick Step Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit is to assay OT/BGP levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to OT/BGP. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for OT/BGP is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain OT/BGP and HRP conjugated OT/BGP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of OT/BGP. You can calculate the concentration of OT/BGP in the samples by comparing the OD of the samples to the standard curve.

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