Aliases:	SMC3; Structural Maintenance Of Chromosomes 3; SMC3L1; HCA; BAM; Bamacan; CSPG6; Basement Membrane-Associated Chondroitin Proteoglycan; Structural Maintenance Of Chromosomes Protein 3; Chondroitin Sulfate Proteoglycan 6 (Bamacan); Chromosome-Associated Polypeptide; SMC Protein 3; BMH; Chondroitin Sulfate Proteoglycan 6; Bamacan Proteoglycan; CDLS3; SMC-3
Background:	UniProt Entry: Q9UQE7;NCBI Gene Entry: 9126
Application Information	Molecular Weight: Predicted, 142 kDa, observed, 141 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2155 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SMC3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of SMC3 expression in HepG2 cells using anti-SMC3 antibody 1:2,000. Green, isotype control; red, SMC3.

Immunocytochemical staining of HepG2 cells with anti-SMC3 antibody 1:1,000. Nuclei were stained blue with DAPI; SMC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-SMC3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-SMC3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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