Aliases:	SMCHD1; Structural Maintenance Of Chromosomes Flexible Hinge Domain Containing 1; KIAA0650; FSHD2; Structural Maintenance Of Chromosomes Flexible Hinge Domain-Containing Protein 1; SMC Hinge Domain-Containing Protein 1; EC 3.6.1.-; BAMS
Background:	UniProt Entry: A6NHR9;NCBI Gene Entry: 23347
Application Information	Molecular Weight: Predicted, 226 kDa, observed, 226 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2385 Spncies Reactivity: Human, Rat Applications Tested: Western Blotting (WB), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SMCHD1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Immunocytochemical staining of HepG2 cells with anti-SMCHD1 antibody 1:1,000. Nuclei were stained blue with DAPI; SMCHD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-SMCHD1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-SMCHD1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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