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Rabbit Anti-RHOG antibody
Rabbit Anti-RHOG antibody
RHOG; RHO G; ARHG; rho-related GTP-binding protein RhoG precursor; rho-related GTP-binding protein RhoG; ras homolog gene family, member G (rho G).
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  • NO.:SL10466R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human(predicted:Mouse,Rat)
    Applications:Flow-Cyt
    concentration:1mg/ml
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Product Name RHOG
Chinese Name RHOG抗体
Alias RHOG; RHO G; ARHG; rho-related GTP-binding protein RhoG precursor; rho-related GTP-binding protein RhoG; ras homolog gene family, member G (rho G).
Research Area transcriptional regulatory factor
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human, (predicted: Mouse, Rat, )
Applications Flow-Cyt=0.2ug/test (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 21kDa
Cellular localization The cell membrane
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human RHOG: 61-150/191
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X. [provided by RefSeq, Aug 2011].

SWISS:
P84095

Gene ID:
391

Database links:

Entrez Gene: 391 Human

Entrez Gene: 56212 Mouse

Entrez Gene: 308875 Rat

Omim: 179505 Human

SwissProt: P84095 Human

SwissProt: P84096 Mouse

Unigene: 501728 Human

Unigene: 259795 Mouse

Unigene: 11976 Rat



Product Picture
Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-RHOG antibody (SL10466R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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