Product Name	DHX30
Chinese Name	ATP-依赖的RNA解旋酶30抗体
Alias	DDX30; DEAH box protein 30; Dhx30; DHX30_HUMAN; FLJ11214; KIAA0890; Putative ATP-dependent RNA helicase DHX30; Ret CoR.
Research Area	Cell biology Developmental biology Cell differentiation Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse(predicted:Human,Rat,Dog,Pig,Cow,Horse,Rabbit)
Applications	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	134kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DHX30: 1-100/194
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013] Subcellular Location: Cytoplasm and Mitochondrion. Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid. Post-translational modifications: Isoform 2 is phosphorylated on Ser-15. Similarity: Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: Q7L2E3 Gene ID: 22907 Database links: Entrez Gene: 22907 Human Entrez Gene: 72831 Mouse Entrez Gene: 367172 Rat GenBank: NM_138614 Human SwissProt: Q7L2E3 Human SwissProt: Q7L2E3-2 Human SwissProt: Q99PU8 Mouse SwissProt: Q5BJS0 Rat Unigene: 517948 Human Unigene: 276305 Mouse Unigene: 163034 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DHX30) Polyclonal Antibody, Unconjugated (SL14313R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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