Product Name	EAR1
Chinese Name	嗜酸性粒细胞阳离子蛋白抗体
Alias	ECP1_MOUSE; Cytotoxic ribonuclease; Eosinophil cationic protein 1; Eosinophil secondary granule ribonuclease 1; EAR-1; Ribonuclease 3-1; RNase 3-1; eosinophil cationic protein 1 precursor; Ear1; ECP 1.
literatures	Specific References (3) | SL1754R has been referenced in 3 publications. [IF=25.841] Xuefeng Fei. et al. Neddylation of Coro1a determines the fate of multivesicular bodies and biogenesis of extracellular vesicles. J Extracell Vesicles. 2021 Oct;10(12):e12153 WB ; Mouse. PubMed:34623756 [IF=5.52] Park, Shin Yong, et al. "Peptidoglycan Recognition Protein 1 Enhances Experimental Asthma by Promoting Th2 and Th17 and Limiting Regulatory T Cell and Plasmacytoid Dendritic Cell Responses." The Journal of Immunology (2013). Mouse. PubMed:23420883 [IF=2.629] Yu Wen-Yan. et al. Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation. Evid-Based Compl Alt. 2022;2022:4571981 IHC ; Rat. PubMed:35069759
Research Area	Cell biology immunology Channel protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	14kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse Eosinophil cationic protein 1: 65-155/155
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Eosinophil derived neurotoxin (EDN) is a protein belonging to the ribonuclease (RNase) A superfamily. It has recently been found to have antiviral activity against respiratory syncytial virus and human immunodeficiency virus in vitro. Function: Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities. Subunit: Interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). In vitro interacts with and insert into lipid bilayers composed of dioleoyl phosphatidylcholine and dioleoyl phosphatidylglycerol. In vitro, tends to form amyloid-like aggregates at pH 3, but not at pH 5, nor 7. Subcellular Location: Secreted. Note=Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus. Similarity: Belongs to the pancreatic ribonuclease family. SWISS: P97426 Gene ID: 13586 Database links: Entrez Gene: 13586 Mouse
Product Picture	Sample: Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Bone tissue lysates Primary: Anti-EAR1 (SL1754R) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 17 kDa Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EAR1) Polyclonal Antibody, Unconjugated (SL1754R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-EAR1 antibody (SL1754R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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