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Product Name p21-ARC Chinese Name 肌动蛋白相关蛋白2/3复合物亚基3抗体 Alias Actin related protein 2/3 complex subunit 3; ARC21; ARPC3_HUMAN; ARP2/3 complex 21 kDa subunit; ARP2/3 protein complex subunit p21; ARPC3. Research Area Cell biology Signal transduction Cytoskeleton Immunogen Species Rabbit Clonality Polyclonal React Species Mouse(predicted:Human,Rat,Chicken,Cow,Rabbit,Xenopus laevis) Applications WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 20kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human p21-ARC: 2-100/178 Lsotype IgG Purification affinity purified by Protein A Buffer Solution Mouse(predicted:Human,Rat,Chicken,Cow,Rabbit,Xenopus laevis)1M TBS(pH7.4) with 1% BSA, Mouse(predicted:Human,Rat,Chicken,Cow,Rabbit,Xenopus laevis)3% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
Function:
p21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS (Strasser et al, 2004).
Subunit:
Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and
Subcellular Location:
Cytoplasm, cytoskeleton. Cell projection.
Similarity:
Belongs to the ARPC3 family.
SWISS:
O15145
Gene ID:
10094
Database links:Entrez Gene: 10094 Human
Entrez Gene: 56378 Mouse
Entrez Gene: 415204 Zebrafish
Omim: 604225 Human
SwissProt: O15145 Human
SwissProt: Q9JM76 Mouse
Unigene: 524741 Human
Unigene: 275942 Mouse
Product Picture Protein: intestinal(mouse) lysate at 30ug;
Primary: rabbit Anti-p21-ARC (SL17589R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000;
Predicted band size:20 kD
Observed band size: 20 kDProtein:
intestinal(mouse) lysate at 30ug;
Raji(human) lysate at 30ug;
Primary: rabbit Anti-p21-ARC (SL17589R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000;
Predicted band size:20 kD
Observed band size: 20 kDSample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-p21-ARC (SL17589R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- p21-ARC (SL17589R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p21-ARC) Polyclonal Antibody, Unconjugated (SL17589R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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