Product Name	CD163
Chinese Name	CD163抗体
Alias	Scavenger receptor cysteine-rich type 1 protein M130; CD 163; CD163 antigen; CD163 molecule; Hemoglobin Scavenger Receptor; M130; M130 antigen precursor; Macrophage associated antigen; MM130; C163A_HUMAN.
Research Area	Cardiovascular immunology The cell membraneReceptor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,Mouse(predicted:Rat)
Applications	WB=1:500-2000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	121kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD163: 551-650/1156 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] Function: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. Subcellular Location: Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells. Tissue Specificity: Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. Post-translational modifications: A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. Phosphorylated. Similarity: Contains 9 SRCR domains. SWISS: Q86VB7 Gene ID: 9332 Database links: Entrez Gene: 9332 Human Entrez Gene: 93671 Mouse Entrez Gene: 312701 Rat Omim: 605545 Human SwissProt: Q86VB7 Human SwissProt: Q2VLH6 Mouse Unigene: 504641 Human Unigene: 37426 Mouse Unigene: 203204 Rat CD163 is a member of the SRCR superfamily, also known as hemoglobin scavenger receptor (HbSR), M130, or p155. The expression level of CD163 is regulated by many factors; glucocorticoids and anti-inflammatory mediators such as IL-10 and IL-6 can upregulate CD163, whereas pro-inflammatory mediators such as lipopolysaccharide (LPS), γ-Interferon (interferon-γ, IFN-γ), and TNF-α inhibit CD163 expression. CD163 is a type I membrane protein, also known as M130 antigen, Ber-Mac3, Ki-M8, or SM4. CD163 is restrictedly expressed in the monocyte/macrophage lineage; all circulating monocytes and macrophages in most tissues (except the mantle zone and germinal center of lymphoid follicles) are positive, and it is mainly used for the detection of monocytes/macrophages.
Product Picture	Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-CD163 (SL23127R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 130 kD Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-CD163 (SL23127R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 130 kD Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: HL60 (Human) Cell Lysate at 30 ug Primary: Anti-CD163 (SL23127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kD Observed band size: 110 kD