Product Name	Anti-Fas Ligand/AF594
Chinese Name	AF594标记的FasL抗体
Alias	APO1L; Apoptosis (APO 1) antigen ligand 1; Fas-L; Apoptosis antigen ligand 1; APTL; CD178; CD-178; CD 178; CD95L; CD95 ligand; CD95-L; CD95L protein; FAS antigen ligand; Fas L; Fas Ligand; FASL; Fasl; Fas ligand (TNF superfamily member 6); Generalized lymphoproliferative disease; TNF superfamily member 6; TNFSF6; Tumor necrosis factor ligand superfamily member 6; TNFL6_HUMAN; Apoptosis antigen ligand; Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble form; FASLG.
Research Area	Tumour Cell biology immunology Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,Mouse,Rat(predicted:Cow)
Applications	Flow-Cyt=1μg/Test,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Molecular weight	31kDa
Form	Lyophilized or Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Fas Ligand
Lsotype	IgG
Purification	affinity purified by Protein A
Storage Buffer	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 1M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Product Detail	background: FasL (CD95L) is a cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T cell mediated apoptosis and in T cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). May be released as type II membrane protein. Belongs to the tumor necrosis factor family Function: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Subunit: Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Secreted. Cytoplasmic vesicle lumen. Lysosome lumen. Note=May be released into the extracellular fluid, probably by cleavage form the cell surface. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Post-translational modifications: N-glycosylated. The soluble form derives from the membrane form by proteolytic processing. Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization. Monoubiquitinated. DISEASE: Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. Similarity: Belongs to the tumor necrosis factor family. Database links: Entrez Gene: 356 Human Entrez Gene: 14103 Mouse Entrez Gene: 25385 Rat Omim: 134638 Human SwissProt: P48023 Human SwissProt: P41047 Mouse SwissProt: P36940 Rat Unigene: 2007 Human Unigene: 3355 Mouse Unigene: 9725 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Fas-L is a与TNFRSF6/FAS bind的cell factor，一个转导凋亡but positively regulates PDPK1 kinase activity by enhancing its autophosphorylation and significantly reducing the binding of PDPK1 to 14-3-3 proteins.到cells内的一个Receptor。他可能participates inT-cellsregulates凋亡cells毒性和T-cells的发育。regulates凋亡的TNFRSF6/FAS 在外周组织耐受性方面和抗原刺激成熟lymphocyte自杀中起作用。FasL诱导物ReceptorTNFRSF6B/DcR3bindregulatesIts role。 FasL只表达于activation的lymphocyte，但在小鼠Testis中可见高表达的FasL;The molecular weight is 36-43kDa. It belongs to the TNF family.