Aliases:	CDK4; Cyclin Dependent Kinase 4; PSK-J3; Cell Division Protein Kinase 4; Cyclin-Dependent Kinase 4; EC 2.7.11.22; EC 2.7.11; CMM3
Background:	UniProt Entry: P11802;NCBI Gene Entry: 1019
Application Information	Molecular Weight: Predicted, 34 kDa, observed, 30 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB750 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Cdk4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Cdk4 expression in HepG2 cells using Cdk4 antibody 1:1,000. Nuclei were stained blue with DAPI; Cdk4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Cdk4 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cdk4 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively.

Western blotting analysis using anti-Cdk4 antibody . Cdk4 expression in wild type (WT) and Cdk4 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with anti-Cdk4 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively.