Product Name	Phospho-MKK3(Ser218) + MKK6(Ser207)
Chinese Name	磷酸化丝裂原活化蛋白激酶MKK3/6抗体
Alias	MKK3(Phospho-Ser218); MAP Kinase Kinase 3; Dual specificity mitogen activated protein kinase kinase 3; ERK kinase 3; MAP kinase kinase 3; MAP2K 3; MAPK ERK kinase 3; MAPK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; Mitogen activated protein kinase kinase 3; MKK 3; MKK3; mMKK 3b; mMKK3b; MPK 3; PRKMK 3; PRKMK3; Protein kinase mitogen activated kinase 3; SKK2; zMKK 3; MP2K3_HUMAN; MP2K6_HUMAN
Product Type	Phosphorylated anti
Research Area	Tumour  Signal transduction  Apoptosis  Growth factors and hormones  transcriptional regulatory factor  Kinases and Phosphatases  The cell membrane受体
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	39kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human MKK3 around the phosphorylation site of Ser218: VD(p-S)VA
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. Function: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Subunit: Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3, RAC1 and CCM2. Interacts with ARRB1. Interacts with Yersinia yopJ. Tissue Specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. Post-translational modifications: Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. Phosphorylated by TAOK2. Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. DISEASE: Note=Defects in MAP2K3 may be involved in colon cancer. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain. SWISS: P46734 Gene ID: 5606 Database links: Entrez Gene: 5606 Human Entrez Gene: 26397 Mouse Entrez Gene: 303200 Rat Omim: 602315 Human SwissProt: P46734 Human SwissProt: O09110 Mouse Unigene: 514012 Human Unigene: 18494 Mouse Unigene: 100064 Rat
Product Picture	Sample: Muscle(Rat) Lysate at 40 ug Primary: Anti-Phospho-MKK3(Ser218) + MKK6(Ser207) (SL3274R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 40 kD Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-Phospho-MKK3(Ser218) + MKK6(Ser207) (SL3274R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 40 kD Tissue/cell: human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-MKK3 (Ser218)+MKK6 (Ser207) Polyclonal Antibody, Unconjugated(SL3274R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-MKK3(Ser218) + MKK6(Ser207)) Polyclonal Antibody, Unconjugated (SL3274R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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