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Product Name phospho-IRS-2(Ser731) Chinese Name 磷酸化胰岛素受体底物2抗体 Alias IRS-2(phospho-Ser731); IRS-2(phospho S731); Insulin Receptor Substrate 2; IRS 2; IRS-2; IRS2; IRS2_HUMAN. literatures Specific References (1) | SL5397R has been referenced in 1 publications.[IF=7.376] Wei Yu. et al. Silencing TXNIP ameliorates high uric acid-induced insulin resistance via the IRS2/AKT and Nrf2/HO-1 pathways in macrophages. Free Radical Bio Med. 2022 Jan;178:42 WB ; Human.Product Type Phosphorylated anti Research Area Cardiovascular Cell biology immunology Neurobiology Signal transduction Growth factors and hormones transcriptional regulatory factor Diabetes Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 147kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human IRS-2 around the phosphorylation site of Ser731: AS(p-S)PA Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The family of insulin receptor substrates (IRSs) has been reported to play important roles for signal transduction of various hormones. Four members of the IRS family have been described. Each IRS is believed to have different functions; however, the distinct physiological roles of each IRS are unclear. Summary: This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.
Function:
May mediate the control of various cellular processes by insulin.
Subunit:
Interacts with PHIP.
Subcellular Location:
Cytoplasm, cytosol.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
SWISS:
Q9Y4H2
Gene ID:
8660
Database links:Entrez Gene: 8660 Human
Entrez Gene: 384783 Mouse
Omim: 600797 Human
SwissProt: Q9Y4H2 Human
SwissProt: P81122 Mouse
Unigene: 442344 Human
Unigene: 407207 Mouse
IRS-2是肝胰岛素Signal transduction核心介子,IRS-2基因的缺失或IRS-2信号网络上一些关键信号分子的异常改变,都将导致肝胰岛素Signal transduction能力减弱,出现肝胰岛素抵抗.Product Picture SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-IRS-2(Ser731)) polyclonal Antibody, Unconjugated (SL5397R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-IRS-2(Ser731)) polyclonal Antibody, Unconjugated (SL5397R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-IRS-2(Ser731)) polyclonal Antibody, Unconjugated (SL5397R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control(black line):SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-phospho-IRS-2(Ser731) antibody (SL5397R)
Dilution:1ug/Test;
Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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