Product Name	NARC1/PCSK9
Chinese Name	神经Apoptosis调节转化蛋白1/前蛋白转化酶枯草溶菌素9抗体
Alias	FH3; HCHOLA3; Hypercholesterolemia autosomal dominant 3; NARC 1; NARC-1; NARC1; Neural apoptosis regulated convertase 1; Neural apoptosis-regulated convertase 1; PC 9; PC9; PCSK 9; PCSK9; PCSK9_HUMAN; Proprotein convertase 9; Proprotein convertase PC9; Proprotein convertase subtilisin/kexin type 9; PSEC0052; Subtilisin/kexin like protease PC9; Subtilisin/kexin-like protease PC9.
literatures	Specific References (7) | SL6060R has been referenced in 7 publications. [IF=5.34] Wei Zou. et al. Imperatae rhizoma-Hedyotis diffusa Willd. herbal pair alleviates nephrotic syndrome by integrating anti-inflammatory and hypolipidaemic effects. Phytomedicine. 2021 Sep;90:153644 WB ; Rat. PubMed:34274601 [IF=4.38] Zhu, Wenqiang. et al. Metformin Ameliorates Hepatic Steatosis induced by olanzapine through inhibiting LXRα/PCSK9 pathway. SCI REP-UK. Sci Rep-Uk. 2022 Apr;12(1):1-13 IF ; Human,Mouse. PubMed:35379885 [IF=4.01] Su ZL et al. Aloe-emodin exerts cholesterol-lowering effects by inhibiting proprotein convertase subtilisin/kexin type 9 in hyperlipidemic rats. Acta Pharmacol Sin. 2020 Mar 18. WB ; rat,human. PubMed:32203084 [IF=2.858] R. Yang. et al. Xinnaokang improves cecal microbiota and lipid metabolism to target atherosclerosis. Lett Appl Microbiol. 2021 Dec;73(6):779-792 IHC ; mice. PubMed:34596907 [IF=2.65] Huiming Hu. et al. The Hypolipidemic Effect of Hawthorn Leaf Flavonoids through Modulating Lipid Metabolism and Gut Microbiota in Hyperlipidemic Rats.. EVID-BASED COMPL ALT. 2022 Nov;2022:3033311-3033311 WB ; Rat. PubMed:36425260 [IF=1.55] Jia, Lianqun, et al. "Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats." Molecular Medicine Reports (2016). IHC-P ; Rat. PubMed:27082100 [IF=1.182] Tanaka H et al. Proprotein Convertase Subtilisin/Kexin Type 9 Is Associated with Degenerating Adipocytes in Abdominal Aortic Aneurysm.(2018)J Oleo Sci.Oct 11;67(10):1355-1360. IF ; Human. PubMed:30210081
Research Area	Cardiovascular Cell biology immunology Neurobiology Signal transduction Stem cells Apoptosis transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	57kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PCSK9/NARC1: 231-330/692
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. Function: The precursor protein but not the mature protein may form multimers. Subunit: The precursor protein but not the mature protein may form multimers. Subcellular Location: Secreted. Tissue Specificity: Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. Post-translational modifications: The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. Similarity: Belongs to the peptidase S8 family. Contains 1 peptidase S8 domain. SWISS: Q8NBP7 Gene ID: 255738 Database links: Entrez Gene: 255738 Human Entrez Gene: 100102 Mouse Entrez Gene: 298296 Rat Omim: 607786 Human SwissProt: Q8NBP7 Human SwissProt: Q80W65 Mouse SwissProt: P59996 Rat Unigene: 18844 Human Unigene: 133268 Mouse Unigene: 19195 Rat 前蛋白转化酶枯草溶菌素9(PCSK9)是近年来新发现的一种与调节脂质代谢密切相关的基因,编码前蛋白转化酶NARC-1。Narc-1/pcsk9可通过调节细胞表面的低密度Lipoprotein受体(LDLR)水平,参与脂质代谢,从而在动脉粥样硬化性疾病的发生、发展中发挥重要作用。
Product Picture	Sample: Lane 1: Recombinant human PCSK9 protein, C-His (HEK293)( SL43105P) Primary: Anti-NARC1/PCSK9 (SL6060R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 63 kDa Sample: Lane 1:A431(Human) Cell Lysate at 30 ug Lane 2:Uterus (Mouse) Lysate at 40 ug Primary: Anti- NARC1/PCSK9 (SL6060R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 70 kD Sample: Lane 1: HepG2(Human) Cell Lysate at 30 ug Lane 2: SW480(Human) Cell Lysate at 30 ug Primary: Anti- NARC1/PCSK9 (SL6060R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 70 kD Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1/PCSK9) Polyclonal Antibody, Unconjugated (SL6060R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1 PCSK9) Polyclonal Antibody, Unconjugated (SL6060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1 PCSK9) Polyclonal Antibody, Unconjugated (SL6060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NARC1/PCSK9) polyclonal Antibody, Unconjugated (SL6060R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: K562. Primary Antibody (green line): Rabbit Anti-NARC1/PCSK9 antibody (SL6060R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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