Product Name	G21 protein/NPR2L
Chinese Name	G21蛋白质抗体
Alias	G21 protein; Gene 21 protein; Homologous to yeast nitrogen permease (candidate tumor suppressor); Homologous to yeast nitrogen permease; NPR 2L; NPR L2; NPR like 2; NPR2 like; NPRL 2; NPRL2; Tumor suppressor candidate 4; TUSC 4; TUSC 4 protein; TUSC4; TUSC4 protein; NPRL2_HUMAN.
Research Area	Tumour Cell biology Chromatin and nuclear signals Signal transduction Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat, (predicted: Mouse, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	44kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NPR2L: 221-320/380
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	NPR2L is homologous to yeast nitrogen permease and is a candidate tumor suppressor, being a negative regulator of cell cycle. Most abundant in skeletal muscle, followed by brain, liver, and pancreas, with lower amounts in lung, kidney, placenta, and heart. Expressed in most lung cancer cell lines tested. There are two isoforms, produced by alternative splicing. Function: Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs. Subunit: Forms a heterodimer with NPRL3. Interacts with PDPK1. Tissue Specificity: Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in most lung cancer cell lines tested. Similarity: Belongs to the NPR2 family. SWISS: Q8WTW4 Gene ID: 10641 Database links: Entrez Gene: 508487 Cow Entrez Gene: 10641 Human Entrez Gene: 56032 Mouse Omim: 607072 Human SwissProt: Q5E9U9 Cow SwissProt: Q8WTW4 Human SwissProt: Q9WUE4 Mouse Unigene: 437083 Human Unigene: 11337 Mouse
Product Picture	Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-NPR2L (SL6079R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (G21 protein/NPR2L) Polyclonal Antibody, Unconjugated (SL6079R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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