Product Name	Rubisco
Chinese Name	核糖二磷酸羧化酶大链单克隆抗体
Alias	rbcL; RBL_SPIOL; Ribulose bisphosphate carboxylase large chain; EC:4.1.1.39; RuBisCO large subunit; RuBisCO large subunit [ Spinacia oleracea (spinach) ]; ribulose-1; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) [Arabidopsis lyrata];
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	H1E7
React Species	Arabidopsis Thaliana,Rice
Applications	WB=1:500-2000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	52kDa
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG1/Kappa
Purification	Affinity purified by Protein G
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. Function: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process (PubMed:2928307) (Probable). Both reactions occur simultaneously and in competition at the same active site (Probable). Binds to abscisic acid (ABA) which has weakly inhibits carboxylation and more strongly inhibits enzyme activation (PubMed:26197050). Subunit: Heterohexadecamer of 8 large chains and 8 small chains. Subcellular Location: Plastid, chloroplast. Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover. The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization. Similarity: Belongs to the RuBisCO large chain family. Type I subfamily. SWISS: P00875 Gene ID: 2715621 Database links: Entrez Gene: 2715621 Spinacia oleracea SwissProt: P00875 Spinacia oleracea RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate (the main event in carbon dioxide fixation) and the oxidative cleavage of pentose substrates during photorespiration. Both reactions occur simultaneously and compete at the same active site. It binds to abscisic acid (ABA), and ABA has a weaker inhibitory effect on carboxylation but a stronger inhibitory effect on enzyme activation.

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