Chinese Name	胶原酶V
Storage	-20 ℃
Validity	3 Years
Cas No.	9001-12-1
Titer	125 CDU/mg sold
ALIAS	ClostridiDiPetidase a
Appearance	Dark brown powder
Source	From CLOSTRIDIM HISTOLYTICUM
Solubility	5 mg/ml Water

 

 

Collagonase contains at least 7 protease ingredients, and the molecular weight ranges from 68-130kd. It can be used for the separation of the pancreas, and the connective tissue is separated into a single cell.

Solubility: soluble in TES buffer, hank \u0026#39; s balance salt and earles balance salt and other solutions. The solution can be stored for several months at -20 ° C.

Collagonase (Source Solittectomy) contains at least 7 protease ingredients, and the molecular weight ranges from 68-130kd. It can be used for the separation of the pancreas, and the connective tissue is separated into a single cell. The concentration of digestive cartilage tissue is generally 1-2 mg/ml, and the digestive liver tissue is about 1 mg/ml. However, the specific digestion concentration varies from the species. Many literatures pointed out that colloidal enzymes can digest a variety of tissues. The concentration of collagen enzymes is generally 0.1-5 mg/ml. During the digestion, it can be stirred to promote the digestion of enzymes. In the process of digestion, collagen enzymes can cause a small number of cells to die, and the digestive time is generally 15min to a few hours. If the concentration changes, the digestive time may be longer. The preferred buffer during the digestion is calcium ion and Krebs Ringer Buffer of BSA. In the process of digestion, if a large number of cells die, the amount of enzyme is needed, and 0.5%BSA or 5-10%serum can be added to stabilize the state and digestion of the cells. The sterilization of collaoning is first centrifugal or the insoluble object is removed with 0.8µm filters. Then use 0.2 m filters to filter sterilization. For tissue cultivation applications, collagen enzymes can be prepared with solutions without calcium ions such as Hanks, EBSS, etc.

0.05-0.1 mg/ml collagenase dissolved in 50 mm Tes buffer, PH 7.4 (37 ° C) contains 0.36 mm calcium chloride. The final concentration of the reaction system is 50 mm TES, 0.36 mm calcium chloride, 25 mg collagen, 0.005-0.01 mg collaonase.

The enzyme solution can maintain the activity for 15 hours at 37 ° C under a neutral pH containing 0.3-0.5 mm calcium ions. It can be maintained for several months at -20 ° C.

Remarks: Product information may be optimized and upgraded. Please refer to the actual label information.

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