Chinese Name

蛋白酶抑制剂混合物(哺乳动物样品抽提用, 100X)

 

 

 

Product description:

mammalian cells or tissue extracts contain many endogenous proteases, phosphatase, etc. Modify, which affects subsequent protein detection. Therefore, adding proper proteases, phosphates and other inhibitors to the extract is an effective way to prevent protein degradation and decorative modification.

This product is used for protease inhibitors mixture extracted by mammalian cells or tissue protein, which contains broad spectrum, cysteine, and acid protease inhibitors, and amino peptidase inhibitors. If it is used to detect metal protease activity, EDTA should not be added. The proportion of protease inhibitors (mammalian samples, 100X) and 0.5m EDTA are added to the cracking solution at a ratio of 1: 100, which can be used for extraction of mammalian cells or tissue proteins and effectively suppress protein degradation.

Scope of application:

Various protease activity in mammalian cells or tissue extracts, such as lingicine protease, amino peptide, cysteplate Pinopamininase, sulic acid, and citizenin, metal protease, etc. Suitable for Western blot and immune co -sedimentation to detect phosphate protein, protein kinase activity measurement, etc.

How to use:

1. Mixture of protease inhibitors (100X), add 1: 100 to the cracking solution according to the ratio of 1: 100, mix well It can be used later. The cracking solution containing a protease inhibitor mixture should be available for existing, and it should not be prepared for follow -up use after preparation.

2. According to the need, 0.5m EDTA also added to the cracking solution at a ratio of 1: 100.

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