Purpose

Our Sheep Pregnancy-Associated Glycoprotein (PAG) ELISA Kit is to assay PAG levels in sheep serum, plasma, culture media or any biological fluid.
Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate has been pre-coated with an anti-PAG monoclonal antibody and the detection antibody is a  polyclonal antibody which be biotinylated in this kit. Samples and biotinylated polyclonal antibody are added to the appropriate Microelisa stripplate wells in sequence and washed out with TBS or PBS. Then HRP-Avidin is added to each Microelisa stripplate well. The TMB is used for coloration after the HRP-Avidin has been reacted thoroughly and washed out by TBS or PBS. The TMB will appear blue under peroxidase activity then turn yellow after the addition of the stop solution. The depth of color is positively correlated with the factors to be measured in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PAG. You can calculate the concentration of PAG in the samples by comparing the OD of the samples to the standard curve.

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