Purpose

Our Sheep Interferon epsilon(IFNE)ELISA Kit is to assay IFNE levels in Sheep serum, plasma, culture media or any biological fluid.
Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate has been pre-coated with an anti-IFNE monoclonal antibody and the detection antibody is a  polyclonal antibody which be biotinylated in this kit. Samples and biotinylated polyclonal antibody are added to the appropriate Microelisa stripplate wells in sequence and washed out with TBS or PBS. Then HRP-Avidin is added to each Microelisa stripplate well. The TMB is used for coloration after the HRP-Avidin has been reacted thoroughly and washed out by TBS or PBS. The TMB will appear blue under peroxidase activity then turn yellow after the addition of the stop solution. The depth of color is positively correlated with the factors to be measured in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IFNE. You can calculate the concentration of IFNE in the samples by comparing the OD of the samples to the standard curve.
Note: One antibody molecule can be labeled with multiple biotin molecules, and one biotin molecule can bind a horseradish peroxidase labeled avidin, thus bringing a large amount of horseradish peroxidase bound to the antibody, which has higher sensitivity and signal amplification effect than the traditional direct horseradish peroxidase labeled antibody.

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