Measurement Significance:

α-GC (EC 3.2.1.20) is widely present in animals, plants, microorganisms, and cultured cells, catalyzes α between hydrolyzate aryl or hydrocarbyl groups and glycosyl groups.- Glycoside bonds to form glucose, not only related to the relaxation or reinforcement of the cell wall, but also closely related to cell recognition and some signal molecules.

Measurement principle:

α-GC Decomposition - Nitrobenzene-α-D gyladin is a pair-nitrophenol, the latter has the maximum absorption peak at 400 nm, by measuring the absorption value rising high rateCalculate α-GC activity.

Need instruments, consumables:

Visible spectrophotometer / enzyme label, desktop centrifuge, water bath, adjustable pipette, trace quantum-based cuvette / 96-well plate, mortar, ice and distilled water.

 

 

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