Measurement Significance: β-GC (EC 3.2.1.21) is widely present in animal, plant, microorganisms, and cultured cells, catalyzes β-glycosidic hydrolysis, and has multiple physiological effects:In the saccharification of cellulose, β-GC is responsible for further hydrolyzing cellulose diose and cellulose oligosaccharide to generate glucose; β-GC hydrolyzed terpene aroma precursor, so that the glycoside bonds becomes a free state.Thereby producing fragrance; β-GC can hydrolyze plant in the inner wild black cherry, release HCN, thereby preventing insects from feeding.

 

Determination principle: β-GC decomposition pair-nitrobenzene-β-D-gyranoside is to form a maximum absorption peak at 400 nm, by measuring the absorbance valueTo calculate β-GC activity.

Need instruments, consumables: visible spectrophotometer / enzyme label, desktop centrifuge, water bath, adjustable pipette, trace quantum-based cuvette / 96-well plate, mortar, ice and distilled water

 

 

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