DESCRIPTION

Used for the quantitative determination of Anti-MPXV M1R Human IgG concentration in serum and plasma.

PRINCIPLE OF THE ASSAY

This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant MPXV M1R has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-MPXV M1R Human IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-MPXV M1R Human IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.

PRECISION

Intra-Assay Precision (Precision within an assay): <10% Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision. Inter-Assay Precision (Precision between assays): <15% Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

Sample		1	2	3	1	2	3
n		16	16	16	24	24	24
Mean (ng/mL)		8.4	1.5	0.4	8.9	2.3	0.6
Standard deviation		0.6	0.1	0.0	0.8	0.2	0.1
CV \(%\)		6.8	5.5	3.6	8.7	7.5	8.5

RECOVERY

The recovery of Anti-MPXV M1R Human IgG spiked to three different levels in human serum samples throughout the range of the assay was evaluated.

Sample	Dilution factor	Average (%)	Range (%)
Human serum (n=3)	1:1000	93	83-108
	1:2000	97	86-105

LINEARITY

To assess the linearity of the assay, human serum samples were spiked with high concentrations of Anti-MPXV M1R Human IgG and diluted with the Standard Diluent to produce samples with values within the dynamic range of the assay.

Sample	Dilution factor	Average (%)	Range (%)
Human serum (n=5)	1:2	96	94-101
	1:4	99	95-107
	1:8	100	97-104
	1:16	101	93-110

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)