Product Name	Recombinant Rabbit Anti-LGMN Monoclonal Antibody
Cat No.	SLA2123M
Chinese Name	半胱氨酸蛋白酶重组兔单抗
Alias	AEP; LGMN1; PRSC1; LGMN_HUMAN; LGMN; Asparaginyl endopeptidase (AEP); Protease, cysteine 1; 3.4.22.34; LGMN_MOUSE; LGMN_RAT; Asparaginyl endopeptidase; legumain; protease, cysteine, 1 (legumain)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	12C11
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Legumain
Purification	affinity purified by Protein A
Predicted MW.	49 kDa
Observed MW.	35-55 kDa
Subcellular Location	Cytoplasm
Gene ID	5641
Swiss-Prot	Q99538
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Has a strict specificity for hydrolysis of asparaginyl bonds.Can also cleave aspartyl bonds slowly, especially under acidic conditions.Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Legumain monoclonal antibody, unconjugated (SLA2123M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.