DESCRIPTION

Used for the quantitative determination of Anti-Adalimumab concentration in serum and plasma.

PRINCIPLE OF THE ASSAY

This assay employs the quantitative sandwich enzyme immunoassay technique. Adalimumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Adalimumab will be captured by immobilized Adalimumab. After washing away any unbound substances, a biotin-labeled Adalimumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Adalimumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

PRECISION

Intra-Assay Precision (Precision within an assay): <10% Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision. Inter-Assay Precision (Precision between assays): <15% Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

Sample		1	2	3	1	2	3
n		16	16	16	24	24	24
Mean (ng/mL)		46.5	11.0	2.8	50.2	12.4	3.0
Standard deviation		1.8	0.9	0.3	7.2	0.7	0.3
CV \(%\)		3.9	8.4	10.1	14.3	5.4	10.9

RECOVERY

The recovery of Anti-Adalimumab spiked to three different levels in human serum samples throughout the range of the assay was evaluated.

Sample	Dilution factor	Average (%)	Range (%)
Human serum (n=3)	1:10	104	91-115
	1:20	92	82-102

LINEARITY

To assess the linearity of the assay, human serum samples were spiked with high concentrations of Anti-Adalimumab and diluted with the Standard Diluent to produce samples with values within the dynamic range of the assay.

Sample	Dilution factor	Average (%)	Range (%)
Human serum (n=3)	1:2	97	92-100
	1:4	100	98-103
	1:8	101	94-109
	1:16	105	91-117

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)