DESCRIPTION

Used for the quantitative determination of Anti-Denosumab neutralizing antibody in serum and plasma.

PRINCIPLE OF THE ASSAY

This kit employs the quantitative competitive enzyme immunoassay technique for the detection of neutralizing antibodies against Denosumab. Samples are incubated in wells pre-coated with Denosumab; neutralizing antibody in the sample competes with the detection system for binding. After incubation and washing, a substrate solution is added and the color developed is inversely related to the amount of neutralizing antibody present. The reaction is stopped and the optical density is measured.

SPECIFICATIONS

Catalog No.	ADA0003
Size	96 T
Detection method	Colorimetric
Assay type	Quantitative, competitive ELISA
Sample type	Serum, Plasma
Range	15.625-1,000 ng/ml
Shipping	2 - 8℃
Storage	2 - 8℃
Validity	6 months

PRECISION

Intra-assay and inter-assay coefficients of variation: CV < 20%.

RECOVERY

Recovery ranged from 80% to 120%.

STABILITY AND STORAGE

Shipping condition 2 - 8℃. Store the kit at 2 - 8℃ upon receipt; stability of the kit is determined by the loss rate of activity, which is less than 20% over the shelf life.

PRECAUTIONS

1. This product is for research use only. It is not intended for diagnostic or therapeutic use.

2. Bring all reagents and samples to room temperature before use. Refrigerate reagents immediately after use.

3. Avoid repeated freeze-thaw cycles of samples and standards.

4. Wear protective gloves and follow standard laboratory safety procedures.