Product Name	Recombinant Rabbit Anti-MSH2 Monoclonal Antibody
Cat No.	SLA0703M
Chinese Name	错配修复蛋白2单克隆抗体
Alias	COCA1; FCC1; HNPCC; HNPCC1; LCFS2; LYNCH1; MMRCS2; MSH-2; hMSH2; MSH2_HUMAN; MSH2; MutS protein homolog 2; MSH2_MOUSE; MSH2_RAT; mutS homolog 2; mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1); mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli); DNA mismatch repair protein Msh2
Host Species	Mouse
Isotype	IgG1
Clonality	Monoclonal
Clone No.	4C2
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Purified His-tagged MSH2 protein was used to produced this monoclonal antibody.
Purification	affinity purified by Protein A
Concentration	1mg/ml
Subcellular Location	Nucleus
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.