Product Name	MBD1 Recombinant Rabbit mAb
Cat No.	SLM-63074R
Chinese Name	甲基化CpG结合蛋白重组兔单抗
Alias	CXXC3; PCM1; RFT; MBD1_HUMAN; MBD1; CXXC-type zinc finger protein 3; Methyl-CpG-binding protein MBD1; Protein containing methyl-CpG-binding domain 1; methyl-CpG binding domain protein 1; protein containing MBD 1
Host Species	Rabbit
Isotype	IgG
Clonality	Recombinant
Clone No.	18A16
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human MBD1
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	67 kDa
Observed MW.	70-75 kDa
Subcellular Location	Nucleus
Gene ID	4152
Swiss-Prot	Q9UIS9
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.