Product Name	Recombinant Rabbit Anti-CDH5 Monoclonal Antibody
Cat No.	SLA3283M
Alias	7B4; CD144; VE-Cad; VECD; VEcad; Vec; CADH5_HUMAN; CDH5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CADH5_MOUSE; cadherin 5; cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin 5, type 2 (vascular endothelium); VE-cadherin; Cadherin-5; Vascular endothelial cadherin; CD antigen CD144
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7E2
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) ELISA (1:5000-10000) IP (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Purification	affinity purified by Protein A
Gene ID	1003
Swiss-Prot	P33151
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015],
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDH5 | CD144/VE-cadherin Monoclonal Antibody, Unconjugated (SLA3283M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDH5 | CD144/VE-cadherin Monoclonal Antibody, Unconjugated (SLA3283M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDH5 | CD144/VE-cadherin Monoclonal Antibody, Unconjugated (SLA3283M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDH5 | CD144/VE-cadherin Monoclonal Antibody, Unconjugated (SLA3283M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.