Product Name	Recombinant Rabbit Anti-S1PR3 Monoclonal Antibody
Cat No.	SLA2842M
Chinese Name	内皮分化型G蛋白偶联受体3重组兔单抗
Alias	S1PR3_HUMAN; S1PR3; S1P receptor 3; S1P3; Endothelial differentiation G-protein coupled receptor 3; Sphingosine 1-phosphate receptor Edg-3 (S1P receptor Edg-3); C9orf108; C9orf47; EDG3; S1PR3_MOUSE; Lysophospholipid receptor B3; Lpb3; sphingosine-1-phosphate receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; chromosome 9 open reading frame 47; chromosome 9 open reading frame 108
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9E8
React Species	Human
Applications	WB (1:1000-2000) IHC-P (1:100-200) IHC-F (1:100-200) IF (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human EDG3
Purification	affinity purified by Protein A
Predicted MW.	42 kDa
Observed MW.	42 kDa
Gene ID	1903
Swiss-Prot	Q99500
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EDG3 Monoclonal Antibody, Unconjugated(SLA2842M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EDG3 Monoclonal Antibody, Unconjugated(SLA2842M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EDG3 Monoclonal Antibody, Unconjugated(SLA2842M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EDG3 Monoclonal Antibody, Unconjugated(SLA2842M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EDG3 Monoclonal Antibody, Unconjugated(SLA2842M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.