Product Name	Recombinant Rabbit Anti-PAK1&PAK2&PAK3 (p-S144/S141/S139) Monoclonal Antibody
Cat No.	SLA2798M
Chinese Name	磷酸化p21激活激酶1/2/3重组兔单抗
Alias	IDDMSSD; PAKalpha; alpha-PAK; p65-PAK; PAK-1; Paka; PAK1_HUMAN; PAK1; p21-activated kinase 1 (PAK-1); 2.7.11.1; PAK1_MOUSE; CDC42/RAC effector kinase PAK-A; PAK1_RAT; Protein kinase MUK2; p68-PAK; p21 (RAC1) activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21 protein (Cdc42/Rac)-activated kinase 1; STE20 homolog, yeast; PAK1 & PAK2 & PAK3 (phospho-S144/S141/S154); p-PAK1 & PAK2 & PAK3; phospho-PAK1 & PAK2 & PAK3; PAK1 & PAK2 & PAK3 (phospho-Ser144/Ser141/Ser154)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9G6
React Species	Human,Mouse,Rat
Applications	WB (1:500-5000) IHC-P (1:100-200) IHC-F (1:100-200) IF (1:100-200) Flow-Cyt (1:50-100) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PAK1 around the phosphorylation site of S144
Purification	affinity purified by Protein A
Predicted MW.	58-62 kDa
Observed MW.	60-67 kDa
Gene ID	5058
Swiss-Prot	Q13153
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. MCF-7 (H) cells were treated with or without EGF (1µg/ml) for 10 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) monoclonal antibody, unconjugated (SLA2798M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated(SLA2798M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated(SLA2798M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated(SLA2798M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated(SLA2798M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.