Product Name	Recombinant Rabbit Anti-NCF2 Monoclonal Antibody
Cat No.	SLA1918M
Chinese Name	NADPH氧化酶活化蛋白1重组兔单抗
Alias	NCF-2; NOXA2; P67-PHOX; P67PHOX; NCF2_HUMAN; NCF2; 67 kDa neutrophil oxidase factor; NADPH oxidase activator 2; Neutrophil NADPH oxidase factor 2; NCF2_MOUSE; Q3U5S4_MOUSE; NCF2_RAT; neutrophil cytosolic factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2); chronic granulomatous disease, autosomal 2
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human NOXA2
Purification	affinity purified by Protein A
Predicted MW.	60 kDa
Observed MW.	67 kDa
Gene ID	4688
Swiss-Prot	P19878
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. THP-1 (H) cells were treated with or without PMA (100nM) for 48 h, 25 μg total protein per lane of cell lysates (see on figure) probed with NOXA2 monoclonal antibody, unconjugated (SLA1918M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NOXA2 Monoclonal Antibody, Unconjugated (SLA1918M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NOXA2 Monoclonal Antibody, Unconjugated (SLA1918M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NOXA2 Monoclonal Antibody, Unconjugated (SLA1918M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NOXA2 Monoclonal Antibody, Unconjugated (SLA1918M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.