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Product Name Recombinant Rabbit Anti-MSMB Monoclonal Antibody Cat No. SLA1785M Chinese Name 前列腺分泌蛋白重组兔单抗 Alias HPC13; IGBF; MSP; MSPB; PN44; PRPS; PSP; PSP-94; PSP57; PSP94; MSMB_HUMAN; MSMB; Immunoglobulin-binding factor (IGBF); Prostate secreted seminal plasma protein; Prostate secretory protein of 94 amino acids (PSP-94 | PSP94); Seminal plasma beta-inhibin; PRSP; microseminoprotein beta; microseminoprotein, beta-; beta-microseminoprotein Host Species Rabbit Isotype IgG Clonality Monoclonal Clone No. 8D3 React Species Human Applications WB (1:500-2000)
IHC-P (1:200-1000)
IHC-F (1:200-1000)
IF (1:200-1000)
IP (1:20-50)
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.Immunogen A synthesized peptide derived from human beta 2 Microseminoprotein Purification affinity purified by Protein A Predicted MW. 13 kDa Observed MW. 15 kDa Gene ID 4477 Swiss-Prot P08118 Storage 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.Background The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Validation Images
Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Prostate Secretory Protein/PSP monoclonal antibody, unconjugated (SLA1785M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein/PSP Monoclonal Antibody, Unconjugated (SLA1785M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein/PSP Monoclonal Antibody, Unconjugated (SLA1785M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein/PSP Monoclonal Antibody, Unconjugated (SLA1785M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein/PSP Monoclonal Antibody, Unconjugated (SLA1785M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.
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