Product Name	Recombinant Rabbit Anti-IGFBP2 Monoclonal Antibody
Cat No.	SLA1747M
Chinese Name	胰岛素样生长因子结合蛋白2重组兔单抗
Alias	IBP2; IGF-BP53; IBP-2; Igfbp-2; mIGFBP-2; pIGFBP-2; BRL-BP; ILGFBPA; IBP2_BOVIN; IGFBP2; IGF-binding protein 2; IBP2_HUMAN; BP2; IBP2_MOUSE; Q91VK7_MOUSE; IBP2_RAT; insulin like growth factor binding protein 2; insulin-like growth factor binding protein 2 (36kD); insulin-like growth factor binding protein 2, 36kDa; insulin-like growth factor binding protein 2
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	2D1
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human IGFBP2
Purification	affinity purified by Protein A
Predicted MW.	35 kDa
Observed MW.	35 kDa
Gene ID	3485
Swiss-Prot	P18065
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with IGFBP2 monoclonal antibody, unconjugated (SLA1747M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IGFBP2 Monoclonal Antibody, Unconjugated (SLA1747M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IGFBP2 Monoclonal Antibody, Unconjugated (SLA1747M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IGFBP2 Monoclonal Antibody, Unconjugated (SLA1747M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IGFBP2 Monoclonal Antibody, Unconjugated (SLA1747M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.