Product Name	Recombinant Rabbit Anti-CEACAM5 Monoclonal Antibody
Cat No.	SLA1496M
Chinese Name	癌胚抗原重组兔单抗
Alias	CD66e; CEA; CEAM5_HUMAN; CEACAM5; Carcinoembryonic antigen (CEA); Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA cell adhesion molecule 5); Meconium antigen 100; CEA cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; carcinoembryonic antigen related cell adhesion molecule 5; carcinoembryonic antigen
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4F13
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-200) IHC-F (1:100-200) IF (1:100-200) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human CEACAM5
Purification	affinity purified by Protein A
Predicted MW.	77 kDa
Observed MW.	180 kDa
Gene ID	1048
Swiss-Prot	P06731
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CEACAM5 monoclonal antibody, unconjugated (SLA1496M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM5 Monoclonal Antibody, Unconjugated(SLA1496M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM5 Monoclonal Antibody, Unconjugated(SLA1496M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM5 Monoclonal Antibody, Unconjugated(SLA1496M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM5 Monoclonal Antibody, Unconjugated(SLA1496M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.