Product Name	Recombinant Rabbit Anti-STMN1 Monoclonal Antibody
Cat No.	SLA0477M
Chinese Name	白血病相关蛋白18/癌蛋白18单克隆抗体
Alias	C1orf215; LAP18; Lag; OP18; PP17; PP19; PR22; SMN; 19k; P18; P19; Pig; Pp18; prosolin; STMN1_HUMAN; STMN1; Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18 (Op18); Phosphoprotein p19 (pp19); Protein Pr22; STMN1_MOUSE; Leukemia-associated gene protein; STMN1_RAT; Pr22 protein; stathmin 1; chromosome 1 open reading frame 215; stathmin 1/oncoprotein 18; oncoprotein 18
Host Species	Mouse
Isotype	IgG
Clonality	Monoclonal
Clone No.	14F6
React Species	Human
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human Stathmin 1
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	17 kDa
Subcellular Location	Cytoplasm
Gene ID	3925
Swiss-Prot	P16949
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with STMN1 Monoclonal Antibody, Unconjugated(SLA0477M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with STMN1 Monoclonal Antibody, Unconjugated(SLA0477M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with STMN1 Monoclonal Antibody, Unconjugated(SLA0477M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with STMN1 Monoclonal Antibody, Unconjugated(SLA0477M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with STMN1 Monoclonal Antibody, Unconjugated(SLA0477M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STMN1 Antibody, Unconjugated (SLA0477M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STMN1 Antibody, Unconjugated (SLA0477M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.