Product Name	Recombinant Rabbit Anti-SLC2A4 Monoclonal Antibody
Cat No.	SLA0955M
Chinese Name	葡萄糖转运蛋白4重组小鼠单抗
Alias	GLUT4; GT2; Glut-4; twgy; GLUT4_BOVIN; SLC2A4; Glucose transporter type 4, insulin-responsive (GLUT-4); Q29RP5_BOVIN; GLUT4_HUMAN; GLUT4_MOUSE; GLUT4_RAT; solute carrier family 2 member 4; solute carrier family 2 (facilitated glucose transporter), member 4
Host Species	Mouse
Isotype	IgG2b
Clonality	Monoclonal
Clone No.	2E5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human GLUT4 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	54 kDa
Observed MW.	50 kDa
Subcellular Location	Cytoplasm
Gene ID	6517
Swiss-Prot	P14672
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with SLC2A4 | GLUT4 monoclonal antibody, unconjugated (SLA0955M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLC2A4 | GLUT4 Monoclonal Antibody, Unconjugated (SLA0955M) at 1:400 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLC2A4 | GLUT4 Monoclonal Antibody, Unconjugated (SLA0955M) at 1:400 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLC2A4 | GLUT4 Monoclonal Antibody, Unconjugated (SLA0955M) at 1:400 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLC2A4 | GLUT4 Monoclonal Antibody, Unconjugated (SLA0955M) at 1:400 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLC2A4 | GLUT4 Monoclonal Antibody, Unconjugated (SLA0955M) at 1:400 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.