Product Name	Recombinant Rabbit Anti-HGD Monoclonal Antibody
Cat No.	SLA2420M
Chinese Name	尿黑酸氧化酶重组兔单抗
Alias	HGD_HUMAN; HGD; Homogentisate oxygenase; Homogentisic acid oxidase; Homogentisicase; 1.13.11.5; HGO; homogentisate 1,2-dioxygenase; homogentisate 1,2-dioxygenase (homogentisate oxidase); homogentisate oxidase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3A11
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human HGD
Purification	affinity purified by Protein A
Predicted MW.	50 kDa
Observed MW.	50 kDa
Subcellular Location	Cytoplasm
Gene ID	3081
Swiss-Prot	Q93099
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Catalyzes the conversion of homogentisate to maleylacetoacetate.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with HGD monoclonal antibody, unconjugated (SLA2420M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HGD Monoclonal Antibody, Unconjugated (SLA2420M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HGD Monoclonal Antibody, Unconjugated (SLA2420M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HGD Monoclonal Antibody, Unconjugated (SLA2420M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HGD Monoclonal Antibody, Unconjugated (SLA2420M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-HGD antibody (SLA2420M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.