Summary:

 

Purpose

 

Our Quick Step Human lambda immunoglobulin light chain,λ-IgLC ELISA Kit is to assay λ-IgLC levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to λ-IgLC. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for λ-IgLC is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain λ-IgLC and HRP conjugated λ-IgLC Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of λ-IgLC. You can calculate the concentration of λ-IgLC in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1038Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.052	0.0530
		0.054
S1	480ng/ml	1.969	1.9715
		1.974
S2	320ng/ml	1.272	1.2705
		1.269
S3	160ng/ml	0.785	0.7885
		0.792
S4	80ng/ml	0.474	0.4785
		0.483
S5	40ng/ml	0.215	0.2190
		0.223

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)